High-Resolution Radioluminescence Microscopy for the Study of Prostate Tissue Slice Cell Metabolism and Monitoring of Treatment Response
Technical Report,30 Sep 2014,29 Sep 2015
Leland Stanford Junior University Stanford United States
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Currently, prostate cancers are classified using the Gleason Grade system and immunohistochemistry. The shortcoming of this method is that it requires fixation, which kills the cells in the tissue sample. We want to develop an optical microscopy method that can provide a high resolution a few cells image of the local metabolism in prostate cancer tissue slice cultures TSCs. Our hypothesis is that the local glucose concentration in prostate TSCs is correlated to the malignancy of the disease. We aim at developing a microscopy technology to image the uptake of radiotracers into prostate TSCs with a high resolution. This will yield additional information on the metabolic activity of the tissue, which may be used to improve staging of a biopsy or to study the uptake of new tracers in vivo.