Rescue of TET2 Haploinsufficiency in Myelodysplastic Syndrome Patients Using Turbo Cosubstrate
Technical Report,01 Jun 2014,19 Jun 2015
University of Missouri System Kansas City United States
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We continued with our focus on the large scale expression of TET2 dioxygenase. During this period we developed a convenient assay for TET2 dioxygenase, which will be utilized to determine the kinetics of wild-type and various clinical mutants of TET2 dioxygenase. Further, we made a library of putative 2-oxoglutarate analogs which will be used to rescue the activity of TET2 mutants from MDS patient. Initial results shows that the activity of TET2 can be modified using 2-oxoglutarate analogs.