Accession Number:

AD1007201

Title:

Rescue of TET2 Haploinsufficiency in Myelodysplastic Syndrome Patients Using Turbo Cosubstrate

Descriptive Note:

Technical Report,01 Jun 2014,19 Jun 2015

Corporate Author:

University of Missouri System Kansas City United States

Personal Author(s):

Report Date:

2015-07-01

Pagination or Media Count:

20.0

Abstract:

We continued with our focus on the large scale expression of TET2 dioxygenase. During this period we developed a convenient assay for TET2 dioxygenase, which will be utilized to determine the kinetics of wild-type and various clinical mutants of TET2 dioxygenase. Further, we made a library of putative 2-oxoglutarate analogs which will be used to rescue the activity of TET2 mutants from MDS patient. Initial results shows that the activity of TET2 can be modified using 2-oxoglutarate analogs.

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE