DID YOU KNOW? DTIC has over 3.5 million final reports on DoD funded research, development, test, and evaluation activities available to our registered users. Click
HERE to register or log in.
Accession Number:
AD1007185
Title:
Effects of Surface Passivation on Gliding Motility Assays
Descriptive Note:
Journal Article
Corporate Author:
University of New Mexico Albuerqueque United States
Report Date:
2011-06-03
Pagination or Media Count:
8.0
Abstract:
In this study, we report differences in the observed gliding speed of microtubules dependent on the choice of bovine casein used as a surface passivator. We observed differences in both speed and support of microtubules in each of the assays. Whole casein, comprised of as1, as2, b, and k casein, supported motility and averaged speeds of 96667 nms. Alpha casein can be purchased as a combination of as1 and as2 and supported gliding motility and average speeds of 94964 nms. Beta casein did not support motility very well and averaged speeds of 870630 nms. Kappa casein supported motility very poorly and we were unable to obtain an average speed. Finally, we observed that mixing alpha, beta, and kappa casein with the proportions found in bovine whole casein supported motility and averaged speeds of 96666 nms.
Distribution Statement:
APPROVED FOR PUBLIC RELEASE