DNA-Crosslinked micelles as Programmable Materials for Biosensing and Responsive Drug Delivery
Technical Report,01 Sep 2011,31 Aug 2015
University of Utah Salt Lake City United States
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The overarching goal of this research is to develop DNA amphiphiles as stimuli-responsive materials, capable of releasing guest molecules in response to specific chemical or biological stimuli. Research during the project period focused on evaluating two DNA amphiphile architectures. Dendrimeric amphiphiles having three DNAstrands on each monomer were explored, as the DNA strands are capable of forming noncovalent crosslinks to stabilize the micellearchitecture. We did observe a reduced CMC for crosslinked micelles however, the change compared with non-crosslinked micelles was not as large as desired. Thus, we shifted our research plan to focus on controlling guest release by altering the DNA polymer ratio of ourmonomers. This was accomplished by hybridizing or removing a complementary DNA strand on monomeric DNA amphiphiles. We have shown guest diffusion is 63-fold faster when the complementary DNA strand is not attached. We were also curious to explore the effect of amphiphiles on the function of nucleic acid aptamers. We found that small-molecule-binding aptamers were able to function in the presence of non-ionic or anionic surfactants at concentrations above the CMC value with only a small change in binding affinity for the specified target molecule.
- Genetic Engineering and Molecular Biology