3,3'-Dioctadecyloxacarbocyanine perchlorate (DiO) as a Fluorogenic Probe for Measurement of Critical Micelle Concentration
Journal Article - Open Access
University of Utah Salt Lake City United States
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Critical micelle concentration CMC is a key factor in applications of amphiphilic molecules, as it dictates the assembly state of the molecules. Nile Red NR is often used as a fluorogenic dye for the measurement of CMC values, but we have observed that NR is prone to aggregation, which leads to reduced reliability in CMC measurements. Here we evaluate 3,3-dioctadecyloxacarbocyanine perchlorate DiO as an alternative fluorogenic dye for the measurement of CMC values. Both NR and DiO provide values comparable to those reported in the literature. However, we demonstrate that DiO provides improved consistency and is more user friendly than NR for measurement of CMC values.
- Atomic and Molecular Physics and Spectroscopy