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A Novel Field Deployable Point of Care Diagnostic Test for Cutaneous Leishmaniasis

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Technical Report,30 Sep 2014,29 Sep 2015

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University of Texas Medical Branch, Galveston Galeston United States

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Leishmaniasis is caused by the protozoan Leishmania and is generally transmitted by the bite of sand flies of the genus Lutzomyiaor Phlebotomus, The disease has significant global impact, producing 10-20 million cases of leishmaniasis worldwide. Cutaneous leishmaniasisCL is characterized by chronic skin ulcers that can impact the individuals functional status, lead to expensive and untimely treatment, and result in disfiguring scarring. Military training and combat operations resulted in cases of CL in soldiers USA, UK deployed to Central America.More recently 2003-2004, CL was reported in almost 1,200 members of the U.S. Armed Forces deployed to Iraq and Afghanistan, and theinfection is an ongoing concern in the OEFOIF veteran population. To date, there is no field-standardized molecular method based on sensitiveDNA amplification coupled with Lateral Flow reading to detect leishmaniasis. Isothermal amplification by RPA Recombinase PolymeraseAmplification is a novel strategy to diagnose infectious diseases that can be used at the POC because it is highly sensitive, fast, inexpensiveand able to work at most ambient temperatures.

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