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Exploring the Presence of microDNAs in Prostate Cancer Cell Lines, Tissue, and Sera of Prostate Cancer Patients and its Possible Application as Biomarker

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Technical Report,01 Aug 2014,31 Jul 2015

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University of Virginia CHARLOTTESVILLE United States

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Along with colleagues in lab, I have recently discovered a new type of extra-chromosomal circular DNAeccDNAs, also called microDNA in mouse tissue as well as in mouse and human cell lines 1. These eccDNAs are mostly 100-400 bases long, high in GC content, presence of micro homology at the start and end and arise from tens of thousands of unique genomic loci and could serve as disease biomarkers. Discovering anew biomarker for any cancer, in this case prostate cancer is significant because early detection and accurate prognosis is very important to cure the disease without over treating the many patients who do not have life-threatening disease. In this project I proposed potential of microDNA as cancer biomarker was explored. The circular DNAs are expected to be stable due to absence of free 5 or 3 end resistant to exo-nuclease and could be amplified by PCR based method. Finally microDNA were identified in various human cell lines and were compared with the prostate cancer cell lines. It would be great if we could detect microDNA in circulation and in this project microDNA were also isolated and identified in serum isolated from cancer patients.

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