Role of Ca++ Influx via Epidermal TRP Ion Channels
Technical Report,15 Sep 2014,14 Sep 2015
Duke University Durham United States
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To benefit military veterans with amputations who suffer skin problems on their amputation stumps, this proposal describes mechanistic studies to pave the way for novel methods of improving skin barrier function at the residual limb-prosthetic interface. Signaling systems in skin will be modulated to increase barrier function, attenuate irritant dermatitis, and characterize the underlying signaling mechanisms so that they can become better targets for treatment. Progress in year 2 of the funding period is described in this Annual Progress Report. We maintained all the necessary regulatory approvals from the Durham VA, Duke University IRB and the DoD to conduct the human experimentation. We set up experiments in primary skin cells for mechanical stress, which we found disrupts skin barrier function. We also found that activation of ion channel TRPV4 can re-normalize barrier function of the skin that has been disrupted by mechanical stress. We also found this particular pattern for keratinocytes regulatory volume decrease, as a surrogate of their capability to moisturize.