The Role of the Rab Coupling Protein in ErbB2-Driven Mammary Tumorigenesis and Metastasis
Technical Report,30 Sep 2014,29 Sep 2015
McGill University Montreal, QC Canada
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During the Year-2 we focused in our understanding of the role of the Rab Coupling Protein RCP in ErbB2-mediated lung metastasis formation. To accomplish this, we have derived both proficient and deficient ErbB2 stable expressed cell lines and then performed orthotopic mammary fat pad and tail vein cell injection using the classic immunodeficient NCr athymic mouse model as syngenic recipient. Consistent with our previous in vivo data, the overexpression of RCP led into drastically decrease the number of lung. In contrast, the depletion of RCP using RNA interference approach significantly increase the number of lung metastases.