Identifying microRNAs that Regulate Neuroblastoma Cell Differentiation
Technical Report,01 Sep 2014,31 Aug 2015
University of Texas Health Science Center San Antonio United States
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In this reporting period, we mainly focused on characterizing another novel differentiation-inducing miRNA, miR-449a. We examined the differentiation-inducing function of miR-449a in multiple neuroblastoma cell lines. We have demonstrated that miR-449a functions as an inducer of cell differentiation in neuroblastoma cell lines with distinct genetic backgrounds, including the MYCN amplification status. We further show that miR-449s mimic induces cell cycle exit, induces cell apoptosis, and decreases the capacity of the neuroblastoma cells to form colonies. Our results support the strong tumor suppressive function of miR-449a in neuroblastoma cells, providing the experimental evidence for developing miR-499a-based differentiation therapy for neuroblastoma.