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Howard University Flow Cytometric Sorter For Research and Education

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Technical Report,12 Mar 2014,11 Mar 2015

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Howard University Washington United States

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Howard University s newly acquired Fluorescence Activated Cytometric Sorter FACS has been integrated into the new flow cytometric core facility. The instrument became available and open to the research community on 4202015. Currently, there are 11 researchers that have gone through instrument training i.e. antibody panel setup and sample preparations. In the three months it has been active, six Howard University researchers have used the instrument, with three using the equipment twice per week. The instrument has been integrated with the Accuri C6 Flow Cytometric Analyzer and Life Technologies Countess Automated Cell Counter as ancillary supportive instrumentation.Briefly, researchers first use the Countess to determine the concentration of their samples the countess has interchangeable LED light cubes for visualizing all the fluorescent detection channels that are available on the purchased FACS instrument. Next, the cells are sorted results have yielded consistently 98 purity. Populations are further confirmed using the Accuri C6 that was bundled with the purchase of the FACS instrument. Current researchers using the instrument have sciences that include bone reformation Dazhi Yang, microbial ecologies Patrick Ymele-Leki, cell cycle and cancer onset Sharma Sudha, immunotherapies and antigen presentation biomaterials M. Lipscomb, Kunle Kassim, and gene therapies K. Washington.

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