Recombination and Transmission Studies with Influenza Virus.
Annual progress rept. 1 Jun 73-1 Aug 74,
MOUNT SINAI SCHOOL OF MEDICINE NEW YORK
Pagination or Media Count:
Continued studies of temperature sensitive ts mutants have been productive in more precise definition of the genetic lesion with respect to viral function. Intensive study of two neuraminidase deficient NA- mutants have produced striking evidence of the aggregation of such mutants that occurs apparently as a consequence of failure of the defective enzyme to split neuraminic acid from the particles as they emerge from the infected cell. Comparative studies of two viral variants that are NA and NA- with respect to neuraminidase content and activity have demonstrated interesting differences in the replication of these viruses in different cell systems. Application of viral genetics to improvement of influenza vaccines, isolation and characterization of influenza viral polypeptides, and studies of influenza viral replication and its inhibition are described.