Accession Number:

AD0874422

Title:

Factors Affecting Transformation of Pasteurella Novicida,

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Report Date:

1970-08-01

Pagination or Media Count:

19.0

Abstract:

The requirements and characteristics of Pasteurella novicida transformations in liquid suspensions were studied. Transformation frequencies of 0.1 to 0.3 were routinely obtained when recipient cells were harvested from 16-hour agar plates, and highr than 1 when logarithmic-phase broth-grown cells were used. Calcium ions were essential for transformations. The DNA - dose response curve, kinetics of transformation, and pH optimum for transformations were similar to those of other bacterial transformation systems. The genetic relatedness of P. novicida and Pasteurella tularensis was established by transforming P. novicida mutants with DNA extracted from P. tularensis. Author

Subject Categories:

  • Biology
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE