Accession Number:

AD0874414

Title:

Hemin Binding by Pasteurella Pestis,

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Report Date:

1970-08-01

Pagination or Media Count:

17.0

Abstract:

Fully virulent cells of Pasteurella pestis bind hemin when suspended in a solution of hemin in phosphate buffer. Most of the activity remains following treatments that destroy the integrity of the cells however, much of the activity is lost following certain chemical treatments. Partially virulent and avirulent strains of P. pestis, which are nonpigmented on hemin agar, bind only small amounts of hemin in the liquid system. These strains, in contrast to the virulent strains, bind more hemin following treatments that destroy cell integrity. The mechanism of hemin binding apparently involves a chemical, nonenzymatic reaction with a substance in the surface layer of the virulent cells that may be an amino sugar. The low activity in the nonpigmented cells is possibly due to an additional surface layer that masks most of the hemin-binding sites. Author

Subject Categories:

  • Biochemistry
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE