Accession Number:

AD0871977

Title:

Replication of Venezuelan Equine Encephalomyelitis Virus in Vitro: I. Growth in Suspension Cell Cultures Grown in Serum-Free and Defined Media

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Personal Author(s):

• Tribble, Jr., Henry R.
• Hearn, Henry J.
• Nagle, Jr, Stanley C.

Report Date:

1970-06-01

Pagination or Media Count:

20.0

Abstract:

Various mammalian cells propagated in serum-free and chemically defined media yielded high titers of Venezuelan equine encephalomyelitis VEE virus. Some difference in maximal titers was noted, depending upon the medium employed. Of the two serum-free media tested, lactalbumin hydrolysate medium was more effective than the chemically defined medium in stimulating viral growth. The addition of serum to serum-free cultures at the time of viral inoculation had a pronounced effect characterized first by a delay and then by a burst of viral replication to very high titers. Thus, the levels of Vee virus replication appear to be influenced by a variety of nutritional factors whose mechanisms of action have yet to be elucidated.

Descriptors:

• *TISSUE CULTURE CELLS
• *CULTURE MEDIA
• *VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS
• MAMMALS
• IN VITRO ANALYSIS
• ARBOVIRUSES
• BLOOD SERUM
• NUTRITION
• GROWTH(PHYSIOLOGY)

Subject Categories:

• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE