Structure of Water in Escherichia Coli B
FORT DETRICK FREDERICK MD
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Nuclear magnetic resonance NMR was used to measure the degree of order of water in Escherichia coli B. It was necessary to grow the E. coli B in a special chemically defined medium, which contained very low concentrations of paramagnetic ions. Complex media or general chemically defined media gave bacteria with such high paramagnetic ion concentrations that it was difficult to differentiate between paramagnetic ion broadening and water structure broadening of the NMR water spectrum. Using bacteria grown in the special chemically defined medium, we showed that the water in E. coli B was highly ordered and was very different from free water and from polywater.
- Atomic and Molecular Physics and Spectroscopy