Accession Number:

AD0860422

Title:

An Improved Chemically Defined Culture Medium for Strain L Mouse Cells Based on Growth Responses to Graded Levels of Nutrients

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Personal Author(s):

Report Date:

1969-10-01

Pagination or Media Count:

21.0

Abstract:

Nutritional factors were evaluated for effects on growth of mouse fibroblast cells in suspension in a chemically defined medium. Quantitative requirements for each essential amino acid, choline, inorganic phosphate, iron, and zinc were established. An improved chemically defined medium was formulated on the basis of the findings yielding populations of L cells in excess of 5,000, 000ml without nutrient replenishment. When spent medium was replaced periodically, yields approaching 30 x 10 to the sixth power cellsml were attained. The efficiency of utilization of most amino acids in the new medium appears to be twofold to threefold better than results reported by others.

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE