Accession Number:

AD0860417

Title:

The Growth of Venezuelan Equine Encephalomyelitis Virus in Human Diploid Cell Strain WI-38

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Personal Author(s):

• Reitman, Morton
• Green, Leonard

Report Date:

1969-10-01

Pagination or Media Count:

14.0

Abstract:

We demonstrated that Venezuelan equine encephalomyelitis VEE replicated in and adapted rapidly to human diploid cell strain WI-38. Peak titers of as high as 10 to the 9.9th power MICLD50ml were obtained after a 48- hour incubation period at low passage levels in Eagle basal medium supplemented with 3 calf serum. VEE virus replicated poorly in serum-free medium. Propagation of the virus was accompanied by the production of hemagglutinin and cytopathogenic effect. These studies suggest that the WI-38 strain might be an appropriate source for the production of VEE virus for vaccines.

Descriptors:

• *TISSUE CULTURE CELLS
• *VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS
• PATHOLOGY
• CELLS(BIOLOGY)
• VACCINES
• GROWTH(PHYSIOLOGY)
• CULTURE MEDIA
• AGGLUTININS

Subject Categories:

• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE