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The Growth of Venezuelan Equine Encephalomyelitis Virus in Human Diploid Cell Strain WI-38
FORT DETRICK FREDERICK MD
Pagination or Media Count:
We demonstrated that Venezuelan equine encephalomyelitis VEE replicated in and adapted rapidly to human diploid cell strain WI-38. Peak titers of as high as 10 to the 9.9th power MICLD50ml were obtained after a 48- hour incubation period at low passage levels in Eagle basal medium supplemented with 3 calf serum. VEE virus replicated poorly in serum-free medium. Propagation of the virus was accompanied by the production of hemagglutinin and cytopathogenic effect. These studies suggest that the WI-38 strain might be an appropriate source for the production of VEE virus for vaccines.
APPROVED FOR PUBLIC RELEASE