The Importance of Oxidation-Reduction Potential Levels in the Growth of Tissue Cell Lines, Using Earle's L Cells as a Prototype
FORT DETRICK FREDERICK MD
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In the course of investigations concerning suspension growth of Earles L tissue cells, the authors noticed certain erratic growth patterns arising from no recognizable cause. Of environmental factors such as agitator speed, aeration level, temperature, pH, and oxidation-reduction potential level, the latter presented the greatest problem in that least was known about it, and preliminary work showed its potential importance as a most likely cause of erratic growth patterns. Evidence involving oxidation-reduction potential ORP as an important factor in environmental control is presented and discussed. In particular, evidence is given showing effects of growth under constant ORP levels, as well as the predetermination of growth caused by initial ORP effects even in the absence of subsequent control. Optimal ranges for the cell-medium system studied are given.
- Anatomy and Physiology