Contribution to the Detection of Staphylococcal Enterotoxin. 2. Concerning the Effect of Staphylococcal Enterotoxins on Nematodes (Beitrag zum Nachweis von Staphylokokkenenterotoxin. 2. Ueber die Wirkung des Staphylokokkenenteroxins auf Nematoden)
ARMY BIOLOGICAL LABS FREDERICK MD
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With the goal of developing a biological assay method for staphylococcal enterotoxin, the effect on the development of nematodes of the order Strongyloides, Rhabditis oxycerca de Man and Panagrellus redivivus Goody was examined. After preparation by fasting and treatment with 0.02 - 0.2M Na2HPO4.12 H2O, 750 - 2,250 units per ml of Hylase, or 1 - 5 mg per ml bacterial protease, or with untreated mixed cultures adults and larvae, significant resistance was demonstrated in the case of both nematodes species when they were tested against 20 purified staphylococcal enterotoxin type A as well as B in concentrations from 1,000 to 22,000 micrograms per ml. It is concluded that these animals are unsuitable for the detection of staphylococcal enterotoxin.