Accession Number:

AD0857267

Title:

Chromosome Mapping of Pasteurella pseudotuberculosis by Interrupted Membrane Mating

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Personal Author(s):

Report Date:

1969-06-01

Pagination or Media Count:

14.0

Abstract:

Pasteurella pseudotuberculosis Flac, after growth in brain heart infusion to obtain maximum F piliation, mated on membranes with three different multiple auxotrophs of P. pseudotuberculosis F-. Samples of mating pairs were periodically separated by vigorous mixing and plated on selective media containing nalidixic acid to counderselect against the donor strain. The transfer of six markers was observed within 65 minutes after mating, resulting in a partial chromosome map. Author

Subject Categories:

  • Biology
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE