Identification of Clones of Mammalian Cells by Enzymatic Activity and Isoenzyme Distribution Patterns
FORT DETRICK FREDERICK MD
Pagination or Media Count:
Isoenzyme distribution patterns zymograms and enzymic-specific activities were determined for both suspension and monolayer cultures of the LM cell line, its clone Cl l, and four sub-clones from this clone. Identical zymograms were obtained from monolayer cultures of all clones for all enzymes tested. Suspension cultures of all clones yielded identical zymograms for six hydroxy acid dehydrogenases. However, when grown in suspension culture, reproducible and characteristic differences were found in the esterase zymograms from each clone. The growth pattern and esterase zymograms from sub-clone 1-9 varied more from those of the parent and the other sub-clones than any of the others. Thus, this procedure can be used to identify and characterize tissue cells and their clones in culture. The enzymic-specific activities from all cultures were identical when the cultures were sampled at the same stage in their growth cycle.
- Anatomy and Physiology