Nonviable Vee Hemagglutinin Prepared from Tissue Cultures by Gamma Radiation
FORT DETRICK FREDERICK MD
Pagination or Media Count:
Hemagglutinins HA of Venezuelan equine encephalomyelitis VEE virus were produced in Maitland-type cultures of chicken embryo MTCE and in monolayers of hamster kidney, McCoy, and human diploid strain W1-38 cells. Optimal pH values for the demonstration of Ha in MTCE preparations ranged from 5.65 to 5.85. Exposure of MTCE HA to 8 x 1,000,000 r of gamma rays destroyed the infectivity of the antigen while most of the HA activity was retained. Irradiated HA performed satisfactorily in hemagglutination-inhibition tests of human and animal sera.