Accession Number:

AD0852034

Title:

Nonviable Vee Hemagglutinin Prepared from Tissue Cultures by Gamma Radiation

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Personal Author(s):

• Reitman, Morton

Report Date:

1969-04-01

Pagination or Media Count:

7.0

Abstract:

Hemagglutinins HA of Venezuelan equine encephalomyelitis VEE virus were produced in Maitland-type cultures of chicken embryo MTCE and in monolayers of hamster kidney, McCoy, and human diploid strain W1-38 cells. Optimal pH values for the demonstration of Ha in MTCE preparations ranged from 5.65 to 5.85. Exposure of MTCE HA to 8 x 1,000,000 r of gamma rays destroyed the infectivity of the antigen while most of the HA activity was retained. Irradiated HA performed satisfactorily in hemagglutination-inhibition tests of human and animal sera.

Descriptors:

• *RADIATION EFFECTS
• *VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS
• GAMMA RAYS
• EXPOSURE(PHYSIOLOGY)
• BLOOD SERUM
• INHIBITION
• RADIATION DOSAGE
• TISSUE CULTURE
• AGGLUTININS
• EMBRYONATED EGG TECHNIQUE

Subject Categories:

• Radiobiology
• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE