Accession Number:

AD0850914

Title:

Study of Virulent F. Tularensis Strain in the Tissue Culture in Vitro,

Descriptive Note:

Corporate Author:

ARMY FOREIGN SCIENCE AND TECHNOLOGY CENTER CHARLOTTESVILLE VA

Personal Author(s):

Report Date:

1969-03-04

Pagination or Media Count:

11.0

Abstract:

Bacteria of a highly virulent strain -- F. tularensis No. 503 penetrated into the cytoplasm of fibroblasts of the human embryo in vitro and multiplied in these cells. No digestion of intracellularly-located microbes occurred, as it was established in the case of Gaisky restored vaccine strain No. 15. Within the period of 3 weeks of intracellular survival the microbes retained their normal color and morphology. However, tissue cells evidently produced a bacteriostatic effect on the inhabiting microbes, since intracellular multiplication of the latter was limited. By the end of the observation period there appeared areas of cellular layer regeneration, in which mitotic figures could be observed. Irreversible cellular degeneration was noted in areas of marked microbial accumulations. Author

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE