Oxidative Assimilation of Glucose by Pasteurella Pestis
FORT DETRICK FREDERICK MD
Pagination or Media Count:
A lethal effect of glucose on aerated virulent but not avirulent cultures of Pasteurella pestis at 37C observed by Wessman et al. in 1958 prompted further studies into the patterns of oxidative metabolism of the virulent Alexander strain at 37 and 26C. Bacterial respiration was markedly stimulated by glucose this may be associated under certain conditions with the previously demonstrated toxic effect of glucose. The inability of the pathogen to assimilate substantial quantities of cellular material, as well as its fermentative activity under aerobic conditions may also be involved. At 26C in the presence of added NH3, P. pestis assimilates as much glucose as at 37C, but releases considerably less lactic acid into the supernatant fluids. The data are also consistent with the idea that at least a portion of the cellular material synthesized during oxidative assimilation may be due to the reincorporation of endogenously produced ammonia.