Ammonium Sulfate Coprecipitation Antibody Determination with Purified Staphylococcal Enterotoxins
FORT DETRICK FREDERICK MD
Pagination or Media Count:
The ammonium sulfate coprecipitation technique of Farr was applied to purified enterotoxins of Staphylococcus aureus. Studies with iodine 131-labeled enterotoxins A, B, and C, using 1.6 M ammonium sulfate for coprecipitation, revealed differences in the antigen-binding capacity of normal and immune rabbit sera for the enterotoxins. The ammonium sulfate coprecipitation procedure was more sensitive than agar gel diffusion methods for the determination of antibody to enterotoxin B. Antigen-binding tests with anti-enterotoxin B rabbit serum suggest the presence of similar antigenic determinant groups in all three toxins. Measurable antigen-binding capacities for enterotoxins A, B, and C were detected in sera of normal human subjects. Increased levels of binding antibody to enterotoxin B were detected in three of five accidently exposed human subjects.
- Medicine and Medical Research