Relationship of Physical Factors to the Freezing Damage to Red Cells during Freezing and Thawing.
Progress rept. no. 65, 1 Dec 67-30 Nov 68,
JOHN S SHARPE RESEARCH FOUNDATION BRYN MAWR PA
Pagination or Media Count:
Red cells were frozen with three different procedures 1 modification with lactose 15 in saline, .85 NaCl followed by freezing with dextran 16, dextrose 1, in saline 2 freezing with addition of 15 lactose, 10 glucose, in saline and 3 freezing with addition of 15 lactose in saline. Addition of even a minimal quantity of glycerol reduces the recovery of red cells, immediately and after resuspension in saline or plasma. There is a sharp reduction in the osmotic resistance with all concentration of glycerol, whenever glucose is present when red cells are frozen with plain 15 lactose, there is a nearly normal osmotic resistance with the glycerol concentration up to 1.94. The MCV shows a sharp increase with all concentration of glycerol, with increasing hemolysis. The reduced glutathione of the whole blood remains unchanged after freezing and thawing, but between 46.3 and 42 of the initial amount is found outside the red cells. In plain ACD blood reduced glutathione is actually destroyed as storage progresses. Intracellular magnesium is not affected by freezing and thawing of red cells. In ACD blood magnesium increases during the first week of storage, declines slowly and linearly thereafter, losing 22 of the original amount by the 70th day of storage. Author
- Anatomy and Physiology