Permeability of Serratia Marcescens to Some Inorganic Salts
FORT DETRICK FREDERICK MD
Pagination or Media Count:
The physical interactions between Serratia marcescens and solutions of NaCl, CaCl2, CaI2, NaI, and Na2HPO4 NaH2PO4 were examined. Dilute 0.017 N salt solutions did not cause cells to lose water, as evidenced by the unchanged weight of centrifugally packed cells. The cells preferentially adsorbed the cations and repelled the anions of most salts in these solutions. Concentrated 1.71 N salt solutions markedly reduced the weight and water content of centrifugally packed cells, although these took up considerable amounts of salts. More than 90 of the water in the packed-cell pellets was available for the solution of NaCl at 4.2 to 4.4 concentration. The observation that salts apparently penetrated the entire cells freely and yet caused extensive dehydration was not readily compatible with conventional concepts of solute-induced plasmolysis.