Thin-Layer Chromatography of Amino Acid Derivatives With Kieselgel G. N- (2,4-Dinitrophenyl)-Amino Acids and 3-Phenyl-2-Thiohydantoine
ARMY BIOLOGICAL LABS FREDERICK MD
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Except for DNP-Hypro which was not examined, the DNP-derivatives of all protein-amino acids may be simply separated by three simultaneous chromatograms in different systems. One two-dimensional chromatogram and two one-dimensional chromatograms without protracted balancing, and operating simultaneously, can separate 14 of the 19 PTH-amino acids tested. Aided by a simple oxidation technique and by acid hydrolysis with subsequent thin layer chromatographic identification of the freed amino acid, which is stable with hydrolysis in any case, the missing five substances could be identified without further efforts.