Observations by Means of Immunofluorescence of the Appearance and Evolution of Vaccinal Antigen in Cell Cultures,
ARMY BIOLOGICAL LABS FREDERICK MD
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The use of immunofluorescence has made it possible to distinguish the intracellular viral antigen earlier than possible with other methods of investigation. The antigen appears in the cytoplasm of HeLa cells at 6 hours after infection and in KB and human fibroblast cells after 8 hours. In the cellular nucleus the antigen appears at 24 hours in HeLa and human embryo fibroblast cells and at 16-18 hours in KB cells. The histopathological modifications appear after 12-16 hours in HeLa and KB cells and after 16-18 hours in human embryo fibroblast cells. Cellular inclusions appear at 16-18 hours after infection. The infecting virus has been distinguished in the cellular homogenate at 18 hours after infection. The hemagglutinant property was detected only at 48 hours after infection.