Quantitative Study of the Granulopectic Activity of the Reticuloendothelial System by Intravenous Injection of India Ink into Various Animal Species. 1. Method of Quantitative Study of the Granulopectic Activity of the Reticuloendothelial System by Intravenous Injection of Carbon Particles of Known Dimensions
ARMY BIOLOGICAL LABS FREDERICK MD
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The report describes a method which permits the quantitative evaluation of the granulopectic activity of the reticuloendothelial system in contact with the blood in various animal species. This method consists of introducing, intravenously, a homogenous and standardized suspension of carbon, in order to study the kinetics of its disappearance from the blood and its accumulation in the various organs by means of quantitative measurements. In the rat, during the injection of carbon doses of up to 16 mg per 100 g, the disappearance of the carbon from the blood takes place as a direct function of the injected dose. For these doses only the hepatosplenic territories of the reticuloendothelial system and of the hemopoietic tissue participate in the fixation of carbon granules. In the mouse the rate of disappearance of carbon from the blood is directly proportional to the amount injected, up to the dose of 32 mg per 100 g. The phenomenon of the acceleration of blood cleaning appears in this animal species only at doses that are essentially the double of those used in the rat. However, even at these high doses the phenomenon of acceleration of blood cleansing and the degree of pulmonary fixation of the carbon are more attenuated and irregular in the mouse than in the rat.
- Anatomy and Physiology