On the Practical Usefulness of the Urea Method after Dold for the Isolation of Bacterial Spores, Especially for Determination of Anthrax Spores
ARMY BIOLOGICAL LABS FREDERICK MD
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All procedures which propose the isolation of bacterial spores from bacterial mixtures are also more or less harmful to the spores contained in the mixture. The urea method, according to Dold, is less harmful to bacterial spores than the heating process and the antiformin system. In comparative tests of the usefulness of the three methods in isolating anthrax spores the urea method was superior to the other two procedures under arbitrarily chosen as well as under natural conditions. It offers the advantage of a clear, definite rule, applicable to every test material while data concerning the other two methods fluctuate within a wide range, so that a preliminary determination of optimal conditions would be necessary in every case.