Studies on Vitamin Nutrition of Listeria Monocytogenes
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
Studies relating to this report will permit establishment of a medium of determined composition in which culture of Listeria monocytogenes is possible. The basic medium used is composed of a mixture of 19 amino acids the majority synthetics, dextrose and mineral salts. This medium supplemented with riboflavin, biotin, thiamine and thioctic acid protogen, lipoic acid, pyruvate oxidation factor, acetate substitution factor permits growth in series of the microorganism. Omitting any one of these factors of the medium, growth does not occur, which evidence indicates essential factors for the microorganism under experimental conditions.