PROPERTIES OF AN INFECTIOUS NUCLEIC ACID FRACTION FROM CHICKEN EMBRYOS WHICH HAVE BEEN INFECTED WITH ENCEPHALITIS VIRUS. SECOND COMMUNICATION: BIOLOGICAL PROPERTIES
FORT DETRICK FREDERICK MD
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The multiplication of normal Eastern-Equine-Encephalomyelitis EEE viruses is stimulated in the incubated, fertilized chicken egg by means of nucleic acid fractions which were obtained from infected chicken embryos. Plaques can be produced in tissue cultures from embryonal chicken cells with nucleic acid fractions. The infectiousness of the nucleic acid fractions is subject to various inhibitory influences due to substances which are contained in them, in addition to the infectious, high-molecular RNA. These inhibitors can be partly removed or inactivated by means of alcohol precipitation of RNA and by a treatment with desoxyribonuclease. The infectious RNA in the nucleic acid fractions does not come from the EEE virus elementary particles but rather from a ribonuclease-sensitive virus-specific material which must be present in the infected cells.