Accession Number:

AD0840370

Title:

CULTURING OF R. TSUTSUGAMUSHI IN TISSUE CULTURES,

Descriptive Note:

Corporate Author:

ARMY FOREIGN SCIENCE AND TECHNOLOGY CENTER CHARLOTTESVILLE VA

Report Date:

1968-01-01

Pagination or Media Count:

8.0

Abstract:

The culturing of the causative agents of rickettsial disease in tissue cultures was broadly used for the study of the morphology, growth processes, and multiplication of rickettsiae, for the study of the interrelations of rickettsiae with a cell, and for the accumulation of a great number of agents for the purpose of preparing an antigen. The following conclusions were drawn 1 R. tsutsugamushi strains B15, Gilliam, and B58 multiplied well in interbred L-cells and initially trypsinized fibroblasts of chicken embryo. Rickettsiae appeared on the third day, reaching its maximum around 7 - 9th day 2 There was success in supporting a culture of R. tsutsugamushi by means of subinoubations in an interbred culture of L-cells up to five incubations 3 R. tsutsugamushi strain B15 maintains its pathogenic properties for mice after four incubations on a cell culture 4 Infection of a tissue was a sensitive test for the detection of R. tsutsugamushi in materials stored in dehydrated or frozen state. Author

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE