ON THE SUBVIRAL AGENTS OF RABBIT MYXOMA AND SHOPE FIBROMA VIRUSES
FORT DETRICK FREDERICK MD
Pagination or Media Count:
The results from a study of several characteristics of infective subviral agents which can be separated from the mature particles in the cell lysate from RK cells infected with rabbit myxoma or Shope fibroma viruses are as follows 1 SVA from myxoma virus appeared in the sample after 1, 4 and 6 hours of infection while that from the fibroma virus was found after 1 and 8 hours of infection. Both passed through Millipore filters 50 millimicrons and could be separated completely from the mature particles. 2 Just as with mature viruses, both types of SVA were infectious for RK cultured cells as well as for rabbit skin and their titers were unaffected by DNA-ase. 3 Infectivity of myxoma SVA was markedly reduced by trypsin and was completely neutralized by anti-myxoma rabbit serum. Results from a comparison of thermal stabilities indicated that myxoma SVA was markedly heat labile compared to mature viruses and was easily deactivated upon long storage or even on freezing and thawing.