NITROGENOUS COMPONENTS OF NUTRIENT MEDIUM FOR CULTIVATION OF CLOSTRIDIUM PERFRINGENS TYPE D
ARMY FOREIGN SCIENCE AND TECHNOLOGY CENTER CHARLOTTESVILLE VA
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The report concerns the dynamics of the nitrogenous components of the medium in the growth and toxin-formation of Clostridium perfringens of different toxicity. The following conclusions were drawn 1 toxin formation in Cl- perfringens type D is related to the multiplication of the culture 2 in culturing of toxigenic and weak-toxigenic strains of Cl-perfringens type D on a semi-synthetic medium based on a hybrid hydrolysate of casein, the level of total end protein nitrogen of the culture filtrates diminished, while the residual and amino-nitrogen increased in comparison with a sterile medium 3 in the growth of a toxigenic strain there is a sharp increase in the quality of amino nitrogen in the medium during the period of intensive growth and toxin formation. In the filtrates of a culture of a weak-toxigenic strain, the level of nitrogen during the whole period of culturing was relatively stable. This regularity is related to the increased proteolytic activity of the toxigenic strain.