Accession Number:

AD0838814

Title:

INFECTION OF BACILLUS SUBTILIS 168 WITH BACTERIOPHAGE SP-10 AFTER INDUCTION BY MITOMYCIN C OR ULTRAVIOLET LIGHT,

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Personal Author(s):

• Goldberg, Ivan D.
• Bryan, Theodore

Report Date:

1968-06-01

Pagination or Media Count:

27.0

Abstract:

Strains of Bacillus that harbor defective phage PBSX were insensitive to SP-10V, although the phage adsorbed to these insensitive strains. Strains that did not carry the defective phage were sensitive to SP-10V. B. subtilis 168 ind, which can be transduced by SP-10V but is nonpermissive for this phage, was rendered phage-sensitive after treatment with ultraviolet UV light or mitomycin C. Following induction with UV light, maximal sensitivity to SP-10V was obtained at a multiplicity of infection MOI of approximately 14 with mitomycin C induction, an MOI of approximately 1.0 was required. Phage SP-19V maturation in sensitized cells was followed by plating infected streptomycin-sensitive cells in the presence of streptomycin at various stages during phage development. The latent period was estimated at 60 to 75 minutes. We suggest that the resistance of B. subtilis 168 to SP-10 is controlled, at least in part, by the presence of a defective prophage. Author

Descriptors:

• *BACILLUS SUBTILIS
• SENSITIVITY
• TOLERANCES(PHYSIOLOGY)
• INFECTIOUS DISEASES
• GROWTH(PHYSIOLOGY)
• CELLS(BIOLOGY)
• RESISTANCE(BIOLOGY)
• CULTURE MEDIA

Subject Categories:

• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE