Accession Number:

AD0838771

Title:

AN AUTOMATED HEMAGGLUTINATION TECHNIQUE FOR ESTIMATING THE ACTIVITY OF TYPE A BOTULINUM TOXIN

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Personal Author(s):

• Williams, James B.

Report Date:

1968-06-01

Pagination or Media Count:

19.0

Abstract:

A quantitative relationship between hemagglutination titer and animal toxicity of type A botulinum toxin was demonstrated utilizing a modification of the hemagglutination procedure of Rosenfield et al. Hemagglutination titer was correlated to animal toxicity by measuring hemagglutinin activity of known concentrations of toxic material with an established mouse intraperitoneal LD50 value. The automated procedure employed is accurate and reproducible in the range of 5 to 50 micrograms toxin per ml.

Descriptors:

• *TOXINS AND ANTITOXINS
• *CLOSTRIDIUM BOTULINUM
• AUTOMATION
• TOXICITY
• TEST METHODS
• ACCURACY
• VOLUMETRIC ANALYSIS
• SERODIAGNOSIS
• AGGLUTININS

Subject Categories:

• Toxicology
• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE