AN AUTOMATED HEMAGGLUTINATION TECHNIQUE FOR ESTIMATING THE ACTIVITY OF TYPE A BOTULINUM TOXIN
FORT DETRICK FREDERICK MD
Pagination or Media Count:
A quantitative relationship between hemagglutination titer and animal toxicity of type A botulinum toxin was demonstrated utilizing a modification of the hemagglutination procedure of Rosenfield et al. Hemagglutination titer was correlated to animal toxicity by measuring hemagglutinin activity of known concentrations of toxic material with an established mouse intraperitoneal LD50 value. The automated procedure employed is accurate and reproducible in the range of 5 to 50 micrograms toxin per ml.