Accession Number:

AD0838753

Title:

A RAPID SENSITIVE ASSAY FOR STAPHYLOCOCCAL ENTEROTOXIN AND A COMPARISON OF SEROLOGICAL METHODS

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Personal Author(s):

• Silverman, Sidney J.
• Knott, Allen R.
• Howard, Mary B.

Report Date:

1968-06-01

Pagination or Media Count:

16.0

Abstract:

Reversed passive hemagglutination was used to assay enterotoxin in culture filtrates and in food samples. Using cells preserved with either formaldehyde or pyruvic aldehyde, which were tanned and then sensitized with antitoxin globulin, as little as 0.0007 micrograms of enterotoxin was detectable. The results of hemagglutination tests compared well with those obtained by quantitative precipitin tests or by immunodiffusion, but hemagglutination was 50 to 100 times more sensitive than the immunodiffusion technique. In addition, results of the hemagglutination test are available within a few hours, and neither elimination of interfering proteins from food extracts nor concentration of the sample, both of which are necessary for immunodiffusion, is required for this procedure.

Descriptors:

• *FOOD
• *TOXINS AND ANTITOXINS
• SAMPLING
• FROZEN FOODS
• STAPHYLOCOCCUS
• SERODIAGNOSIS
• BIOASSAY
• ANTIGEN ANTIBODY REACTIONS

Subject Categories:

• Toxicology
• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE