Accession Number:

AD0838752

Title:

A METHOD FOR PREPARING SMALL QUANTITIES OF C14-LABELED STAPHYLOCOCCAL ENTEROTOXIN B

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Personal Author(s):

• Bowden, John P.

Report Date:

1968-06-01

Pagination or Media Count:

10.0

Abstract:

A procedure is described for production from Staphylococcus aureus of small amounts of radioisotopically labeled staphylococcal enterotoxin B for animal metabolism studies. The procedure is a modified scaled-down adaptation of the Schantz method of enterotoxin production. The organism is cultured on medium containing C 14-labeled yeast protein hydrolyzate, and the toxin is purified by adsorption on and elution from Amberlite CG-50 resin columns. The procedure is capable of yielding 12 to 16 mg of C 14-labeled enterotoxin B of high purity 96 to 100 with an isotope concentration of 0.2 microcurie per mg.

Descriptors:

• *TOXINS AND ANTITOXINS
• *STAPHYLOCOCCUS AUREUS
• PREPARATION
• ADSORPTION
• PURIFICATION
• LABELED SUBSTANCES
• RADIOACTIVE ISOTOPES
• ANTIGEN ANTIBODY REACTIONS
• CULTURE MEDIA

Subject Categories:

• Toxicology
• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE