A METHOD FOR PREPARING SMALL QUANTITIES OF C14-LABELED STAPHYLOCOCCAL ENTEROTOXIN B
FORT DETRICK FREDERICK MD
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A procedure is described for production from Staphylococcus aureus of small amounts of radioisotopically labeled staphylococcal enterotoxin B for animal metabolism studies. The procedure is a modified scaled-down adaptation of the Schantz method of enterotoxin production. The organism is cultured on medium containing C 14-labeled yeast protein hydrolyzate, and the toxin is purified by adsorption on and elution from Amberlite CG-50 resin columns. The procedure is capable of yielding 12 to 16 mg of C 14-labeled enterotoxin B of high purity 96 to 100 with an isotope concentration of 0.2 microcurie per mg.