CONTRIBUTION TO THE SEROLOGY OF TULAREMIA. HEMAGGLUTINATION, AGGLUTINATION, PARA-AGGLUTINATION AND AGGLUTINATION ABSORPTION
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
The examination of about 150 serum specimens from different stages of this disease proved that hemagglutinating antibodies may be demonstrated from the 5th day on. In tularemia infections the rise of hemaglutinating antibodies nearly parallels that of the antibodies agglutinating B. tularense. The former usually show titers three to seven dilution steps higher than the latter, but this relation is not a general one. The hemagglutination reactions never showed a prozone which was found in 50 of the bacterial agglutinations in one of the cases tested. During the first days of illness in three out of 29 cases heterologous bacteria, e.g. Br. abortus, have been agglutinated to higher titers than B. tularense. The hemagglutinating titers always surpassed those of the bacterial agglutinins whether B. tularense or heterologous bacteria were used as antigens. Thus, an equivocal serological diagnosis is always possible. This applies also to later stages of the illness. In approximately 50 of the cases Brucells abortus was also agglutinated by the sera. Cross reactions with Br. abortus immune sera occur, but do not cause diagnostic difficulties. In cases of Br. abortus infections, especially during the acute phase, hemagglutinating tularemia antibodies may develop. The hemagglutinating anti-tularemia antibodies never surpassed the titers of Br. abortus agglutinations. In the absorption test specific hemagglutinins behave like bacterial agglutinins.