Accession Number:

AD0837859

Title:

A METHOD OF ANALYZING THE INTESTINAL FLORA BY MEANS OF ANTIBODIES LABELED WITH FLUORESCENCE (LABELING WITH 1-DIMETHYLAMINO-NAPHTHALENE-SULFONIC ACID-5)

Descriptive Note:

Corporate Author:

ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s):

• Petuely, F.
• Lindner, G.

Report Date:

1963-07-01

Pagination or Media Count:

5.0

Abstract:

A quantitative method for the analysis of bacterial mixtures, especially those of the stool was applied to the analysis of infant feces. The principle of the method involves cultivation of the various bacterial species or strains from the stools, production of antisera and their linkage to a fluorescent dye. The labeled antisera are used in differential staining of stool smears. The percentage of specifically stained bacteria on the smear is determined by observation or photography under ultraviolet light, followed by visualization of the same field of view in visible light under dark field illumination. The absolute content of specifically stained organisms in the stool and their growth rate during culture may be established by determining the absolute bacterial count in a chamber and by quantitative studies of cultures. Thus, the method offers information on the value of germ counts in selective media.

Descriptors:

• *ENTEROBACTERIACEAE
• *FLUORESCENT ANTIBODY TECHNIQUES
• FLUORESCENCE
• COUNTING METHODS
• TRANSLATIONS
• IMMUNE SERUMS
• INTESTINES
• WEST GERMANY
• CULTURE MEDIA
• INFANTS

Subject Categories:

• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE