Accession Number:

AD0837468

Title:

CONFIGURATION AND LONGITUDINAL DISTRIBUTION OF DNA MOLECULES IN SOLUTION,

Descriptive Note:

Corporate Author:

ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s):

• Lang, Dimitrij
• Kleinschmidt, Albrecht K.
• Zahn, Rudolf K.

Report Date:

1965-10-18

Pagination or Media Count:

18.0

Abstract:

A diffusion method is described for visualization of DNA molecules which is equivalent to the spreading of a mixed DNA-protein solution. Macromolecules diffusing in a solution of about 5 x 10 to the minus 8th power g DNA per ml are adsorbed by a cytochrome c-film at the air-water interface. Subsequently they are transferred to electron microscopic supports. In the enlarged two-dimensional image the end-to-end distances and the length distribution are measured for different DNA preparations from cod fish, trout, holothuria and T2 phage. The molecular shape is not a random coil or a rod but intermediate on account of electrostatic interaction. Author

Descriptors:

• (*DEOXYRIBONUCLEIC ACIDS
• MOLECULAR STRUCTURE)
• SOLUTIONS(MIXTURES)
• ADSORPTION
• ELECTRON MICROSCOPY
• MORPHOLOGY(BIOLOGY)
• ELECTROSTATICS
• INTERACTIONS
• EQUATIONS
• DIFFUSION
• MOLECULAR WEIGHT
• NETHERLANDS

Subject Categories:

• Biochemistry
• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE