DEMONSTRATION OF ERYTHROCYTES MODIFIED BY LIPO-POLYSACCHARIDES, USING THE FLUORESCENT ANTIGENS TECHNIQUE
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
The fluorescent antibody technique, applied with the indirect method, invariably showed a brilliant green fluorescence in erythrocytes treated with endotoxin and agglutinated with the specific antiserum. The fluorescence was diffuse throughout the entire cell body, and particularly marked on the periphery, so as to produce a pattern of thin rings, almost always continuous and regular. Fluorescence was noted not only in erythrocytes crowded into large clots, but also in the isolated cells. The same appearance was observed in erythrocytes treated and dispersed in among a majority of normal cells, but which could nonetheless be readily identified in both the 1 to 10 and the 1 to 20 dilutions. All controls gave negative results. In agreement with Alexanders findings via the direct method, no difficulty was found in distinguishing between true fluorescence and the weak and non-brilliant fluorescence that was sometimes found in erythrocytes treated with normal serums or with non-specific antiserums. Human and sheep erythrocytes behaved identically. There was no difference in behavior under the two methods of procedure, test-tube or glass, in the fluorescence reaction.
- Medicine and Medical Research