ON THE DEMONSTRATION OF STAPHYLOCOCCUS-ENTEROTOXIN
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
The 9-10 days old chick embryo can be injured and caused to die by the injection of 0.1-0.2 milliliters of an enterotoxin-containing culture filtrate in the allantoic cavity. The enterotoxin content of the filtrate pretreated in the usual way heating at 100C30 minutes is not high enough to kill all the embryos of a test series. Among 42 staphylococcus strains isolated from clinical infections or examinations of staff there were four enterotoxin- formers, of which three belonged to phage group III and one belonged to phage group II. The demonstration of the staphylococcus enterotoxin on 9-10 days old chick embryos with the use of unpurified culture filtrates is only advisable for large test series. In the present form, the procedure only allows for qualitative statements, while the quantitative determination of the enterotoxin content in culture filtrates is not presently possible.