BIOCHEMICAL STUDIES ON THE RICE BLAST DISEASE. (PART 10) BIOCHEMICAL CLASSIFICATION OF PIRICULARIA ORYZAE CAVARA (NO 7) REGARDING THE PRODUCTION OF VITAMIN B GROUP BY P.O.C.
Journal article, part 10, no. 7
ARMY BIOLOGICAL LABS FREDERICK MD
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When D group stock-culture biotine and thiamine-dependent is cultivated in the complete culture medium, containing thiamine for 14 days at 25 deg C, 39.36 - 70.78 of the added thiamine remains in the cultivating solution, and 20.54 - 47.75 transfers into the bacteria body, while 8.90 - 14.88 decomposes during cultivation. When B group stock-culture thiamine-non-dependent is cultivated in the thiamine-free culture medium and C group thiamine-non-dependent, but nicotinic acid-dependent in the thiamine-free but nicotinic acid-added culture medium, they show no sign of thiamine formation. By cultivating the six representative stock-cultures in the synthesized culture medium, it was confirmed that riboflavin, pantothenic acid, folic acid and vitamin B6 group were produced in the cultivating solution. Addition of aspartic acid to A group stock-culture biotine-non-dependent in the biotine-free culture medium resulted in better propagation than in the complete culture medium, while such an effect of aspartic acid addition was almost absent in the D group stock-culture. A group stock-culture shows a greater extent of consumption than B or D when glutamic acid and aspartic acid are provided as carbon sources.