Accession Number:

AD0836697

Title:

USE OF SEPHADEX FOR RAPID PURIFICATION OF FLUORESCENCE STAINED ANTIBODY SOLUTIONS

Descriptive Note:

Journal article

Corporate Author:

ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s):

Report Date:

1963-10-01

Pagination or Media Count:

6.0

Abstract:

Quantitative separation of the unbound fluorescence dye from antibody solutions labelled with fluorescence is achieved by the use of gel filtration with Sephadex G 50. As compared with the previously used dialysis, the purification is terminated in a few minutes and therefore can be done at room temperature. Due to the good possibility of optical control, it permits a regain without loss of the serum conjugate with only negligible decrease of concentration.

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE