USE OF SEPHADEX FOR RAPID PURIFICATION OF FLUORESCENCE STAINED ANTIBODY SOLUTIONS
ARMY BIOLOGICAL LABS FREDERICK MD
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Quantitative separation of the unbound fluorescence dye from antibody solutions labelled with fluorescence is achieved by the use of gel filtration with Sephadex G 50. As compared with the previously used dialysis, the purification is terminated in a few minutes and therefore can be done at room temperature. Due to the good possibility of optical control, it permits a regain without loss of the serum conjugate with only negligible decrease of concentration.