EXPERIMENTS WITH LUMINOL
ARMY BIOLOGICAL LABS FREDERICK MD
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The particularly vigorous chemical luminescence occurring on oxidation of 3-aminophthalic acid hydroazide luminol was discovered by W. Lommel and subsequently investigated in greater detail by several researchers. The oxidizing agent most frequently used was a mixture of sodium hypochlorite and hydrogen peroxide. A good chemical luminescence is obtained when luminol is oxidized with hydrogen peroxide alone in the presence of some hemin as catalyst. It is thus highly indicated that this reaction be utilized for the detection of hydrogen peroxide, and experiments show that the luminol test belongs to the most sensitive reactions for hydrogen peroxide.