ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
Methods of detecting histoplasmosis 1 Preparation from blood, bone marrow, sputum, or intestinal rinsings, stained with Giemsa. 2 Biopsy of lymphatic gland, spleen, liver, and marrow. Histological examination of section of ulcerated skin, mucous membrane, and material obtained during bronchoscopy, or section of granuloma of excised lung tissue. 3 Culture H. capsulatum on Sabourauds medium or on blood agar, where it forms a mycelium with characteristic spores. Material for culture can include blood, puncture of lymphatic gland, spleen, liver, or bone marrow, sputum and stool. The running of parallel bacteriological and histological determinations is recommended. 4 Biological test Inoculation of infectious material in experimental animals mice, rabbits, rats, guinea pigs, where the fungus can be determined in the cells of the reticuloendothelial organs. 5 Histoplasmin reaction. Histoplasmin Lilly is a specific antigen obtained from the filtrate of the fungus culture. Manner of administration A 0.1 m1 solution of histoplasmin in a 1 100 or 1 1000 dilution is administered intracutaneously on the forearm. The result is read after 48--72 hours. A reaction is considered positive if at the point of injection there appears an erythema on a hard, nodular, raised background, at least 5 mm in diameter, and surrounded by an edematous zona of the skin. The histoplasmin test has no side effects. 6 Serological reactions.
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